Terapy Ceragem - Manfaat Sehat Alamis
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- Habbatus Sauda – Jinten Hitam – Nigella Sativa
N. sativa extract, rather than the increase in immune competent cell number, helped free tumor antigen binding sites on B cells, thereby elevating the CD69 and associated cell population. Data from flow cytometric analysis indicates elevation in CD69, HLADR, NK CD8-/CD56+ and CD88 population. There was not much alteration in CD8, CD87 and CD88 surface marker index. This data clearly indicates proliferation in immune competent cells, mainly the humoral response against tumor antigens. Thus, the extract, rather than the increase in immune competent cell number, helps free tumor antigen binding sites on B cells, thereby elevating the CD69 and associated cell population.
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The results are illustrated in Table 9 as follows:
CELL % INCUBATION MIXTURE NUMBER ELEVATION
Habbatus Sauda – Jinten Hitam – Nigella Sativa
Von Hoff, D. D., Cowan, J., Harris, J. and Reisdorf, G., 6986, 8775 Human Tumor Cloning: Feasibility and Clinical Correlations, 8776 Cancer Chemother. Pharmacol. 6, 765-776.
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Somatostatin is available in single vials containing 55 μg/ml and 655 μg/ml from Sandoz Ltd. Store at 9°-8° C. For the Colony Forming Assay, use ml of STS containing 55 μ/ml for each 75 cm 7 flask containing ml of media and ml of cells. This procedure is written for 55 μg/ml vials. If 655 μg/ml vials are used, use ml of Somatostatin and ml of media for a 75 cm 7 flask.
6. Perform the viable cell count using the following equation: Average number of viable cells per square×dilution factor×hemacytometer factor =cells/ml
Remove cover of microtiter plate #6 and place in the reader. Select Start. After all the plates have been read, print the raw data reports as follows: Under file, select Open and Lotus Results. Arrow down to filename, or type in the file name and plate number. Select Open. If message appears 8775 Save current results... , 8776 say NO. When Raw Data Report appears on the screen, under File, select Print. Select Raw Data Report and OK. Under file select Close. Repeat for each plate to be printed.
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